Euphorbia hirta has been widely used by Tribal as traditional medicine in a treatment against infectious pathogens. E. hirtaLinn. is a perennial. diffusion method was used to determine the antibacterial activity on gram negative: Euphorbia hirta plant displayed an excellent antibacterial activity against K. The antibacterial and antifungal effect of Euphorbia hirta was evaluated against a panel of bacteria (Escherichia coli, Psueudomonas aeruginosa, Klebsiella.

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Bound flavonoids extract of root showed best activity against C. Table 2 Antibacterial activity of flavonoids of Euphorbia hirta by disc diffusion assay. The MIC values were taken as the lowest concentration of the extracts in the well of the microtiter plate that showed no turbidity after incubation. In the present investigation lowest MIC value 0. Table 3 Antifungal activity of flavonoids of Euphorbia hirta by disc diffusion assay.

Bonjar G, Farrokhi PR. Excellent activity was shown by free and bound flavonoids euhporbia roots of E. Flavonoids in genotypes of Trifolium subterraneum. Antibacterial activity of traditional Australian medicinal plants. Please review our privacy policy. Support Center Support Center.

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Free and bound flavonoids from root, stem leaf, and fruits of E. Identification Selected extract bound hiirta of stem which showed activity against all microorganisms tested was dissolved in ethyl acetate and applied on silica gel coated 0. Spot of Rf value 0.


Ethyl ether free flavonoid and ethyl acetate fractions bound flavonoids thus obtained were dried in vaccuo and weighed. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3. The growth of fungi A. Antibacterial and antifungal activity was expressed in terms of activity index AI.

These plates were developed in an organic solvent mixture of benzene, acetic acid, and water This article has been cited by other articles in PMC.

The potential for developing antimicrobials from higher plants appears rewarding as it will lead to the development of phytomedicine to act against microbes. TA determination TA is the volume at which the test extract can be diluted with the ability to kill the microorganisms. Free flavonoids of stem showed bioactivity against P. BSAC standardized disc susceptibility testing method. The filtrate was extracted in ethyl acetate and washed with distilled water to neutrality.

Niger J Nat Prod Med. Table 6 Quantity and total activity of free and bound flavonoids of Euphorbia hirta. TA of free flavonoid extract of root was found to be the same for P. Perspectives on New Crops and New Uses.

Antimicrobial activity of flavonoids free and bound of Euphorbia hirta L. Traditional medicine practice amongst the igede people of Nigeria.

The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Footnotes Source of Support: Each filtrate was reextracted successively with petroleum ether fraction Iethyl ether fraction IIand ethyl acetate fraction III using separating funnel. Medicinal Plants in East Africa. Broth microdilution method was followed for determination of MIC values. The sample specimen with No. Subramanian SS, Nagarjan S. In the current investigation E. Pharmacognostical and antibacterial studies of leaf extracts of Euphorbia hirta L.


Full TA calculated against E. Antimicrobial activities and toxicological potentials of crude ethanolic extracts of Euphorbia hirta. A few other solvent system n-butanol: Pharmacological activities of Chelidonium majus L. AI for each extract was calculated [ Table 2 ].

Larvicidal activity of some Euphorbiaceae plant extracts against Aedes aegypti and Culex quinquefasciatus Diptera: Total activity TA of extracts against each sensitive pathogen was also evaluated. This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae.

Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta

Quantifying the bioactivity of the plant extracts during screening and bioassay guided fractionation. MIC was determined for the plant extract showing antimicrobial activity against test pathogens. New antimicrobials of plant origin. Antibacillus activity of some euphkrbia used in traditional medicine of Iran.

Present investigation proves the plant under study to possess broad spectrum antimicrobial activity. Rf values of spots of flavonoid extract obtained in TLC solvent system benzene: Financial assistance provided by UGC is gratefully acknowledged.